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Plaque Assay (for Bacteriophages)
The steps in determination of amount of virus by agar suspension plaque assay. This virus is a kind of bacteriophages. Its host cell is a bacterium.
The plaque is the area (spot) in which one viral particle has initially infected a cell (here, a bacterium) and has replicated itself in the cell producing new viruses. The cell is usually lysed upon the release of these newly produced viruses. The new phages go nowhere, but infect the nearby bacteria. Through many rounds of this life cycle, the spot grows big enough to be seen by naked eyes. (In the video, it looks like a hole in the agar) This spot, therefore, represents one virus at the start of the experiment. By counting the plaque number, the amount of virus in original sample can be calculated.
(Note: The remarkable studies of bacteriophage growth were originally pioneered by Ellis and Delbruck 69 years ago.)
Please rate or make a comment on this video.
Please also, if interested, make a discussion and comment on my blog about phages.
http://www.u-sabai-d.com/Bblog/index.php
Cheers.
วิดีโอนี้อธิบายขั้นตอนสำหรับปฏิบัติการหาปริมาณไวรัสโดยการนับจำนวนการเกิดพลัค พลัคเป็นบริเวณที่ปรากฏการเปลี่ยนแปลงของเซลล์เนื่องจากการติดเชื้อไวรัสโดยลักษณะการเปลี่ยนแปลงที่พบบ่อยคือเซลล์แตกและตายเนื่องจากไวรัสที่ติดเชื้อเข้าไปอาศัยเซลล์ในการเพิ่มจำนวน เมื่อดูเปรียบเทียบกับเซลล์ที่อยู่บริเวณข้างๆจะเห็นว่าบริเวณที่เกิดพลัคในกรณีที่เซลล์ตายจะเป็นวงใส ขนาดของวงจะโตขึ้นเรื่อยๆเมื่อเวลาผ่านไป อย่างไรก็ตามขนาดของวงจะหยุดขยายหรือช้าลงหลังจากแบคทีเรียอยู่ในระยะที่มีการเจริญลดลง ทั้งนี้เพราะเฟจจำลองเพิ่มจำนวนได้ดีในขณะที่แบคทีเรียกำลังเจริญแบ่งตัวดี
Length: 494
Rating: 3.30 (3 ratings)
Tags: pfu plaque assay agar suspension phage bacteriophages titer virus amount determination พลัค ไวรัส ปริมาณ
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Mysto & Pizzi get their first Platinum Plaque!
Hey everyone we're extremely proud to announce that we have received our first platinum plaque for R. Kelly's "Get Dirty" off the "Double Up" album. Not only was this our first major placement but it has earned us our first plaque!. Big thanks go out to Wayne Williams and everyone else who helped us get that placement. We did this video to express how we feel about this great achievment, check it out, leave us some comments, and hopefully we'll have many more plaques with our names on them in the future.
Length: 250
Rating: 5.00 (153 ratings)
Tags: Mysto Pizzi Platinum Plaque R. Kelly Get Dirty Double Up Wayne Williams Jive Records
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Live at Leeds 2 The Plaque
On June 17th 2006 Pete and Roger unveil the Blue Plaque commemorating the LAL recording by The Who in 1970. I was able to board the Magic Bus to Leeds for the ceremony, and the later the concert (LAL2) which was AMAZING!!!
Length: 188
Rating: 4.80 (18 ratings)
Tags: Who Rock Live Leeds Townshend Daltrey liveatleeds2006
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Incident at the Memorial Plaque of Mara Buneva in Skopje
The commemoration of the 79th anniversary of the death of Mara Buneva has turned into a scene of anti-Bulgarian activities in central Skopje. Hooligans, probably fans of Vardar FC, attacked the participants in the memorial service -- Bulgarians from Macedonia and representatives of the Internal Macedonian Revolutionary Organization (VMRO). The attackers threw stones chanting "Bulgarians, tatars", Die, Bulgarians, it's Macedonia here". According to unconfirmed reports, the attack was organized still on Friday, when a Macedonian television channel, Sky Net TV, showed a program during which viewers sent SMS in support of stopping the "event of the Bulgarophils about Mara Buneva". Four or five people were injured in the attack. They got issued medical certificates at the hospital in Skopje. The Bulgarian embassy announced that they were specifying the details of the incident.
Length: 96
Rating: 3.60 (39 ratings)
Tags: Bulgaria Bulgarian Macedonia Makedonija Mara Buneva
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Robin Gibb Plaque
Gibb unveiled the plaque on the facade of the former home of the band's manager and producer Robert Stigwood.
It was where the trio spent much of their time rehearsing and composing songs between 1968 and 1980.
Gibb said it was also where they first met actor John Travolta for the making of the film Saturday Night Fever.
At Saturday's unveiling Gibb said: "It's very special and more so because of losing Maurice in 2003 my twin brother.
Length: 136
Rating: 4.90 (11 ratings)
Tags: Robin Gibb Plaque Robert Stigwood BEE GEES BARRY MAURICE
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mucous.colon cleanse,mucoid plaque
Been fasting for 6 days and doing a colon cleanse. The crap/ Stomach Mucous that continue to come out is unbelievable! Also, the energy you get from fasting is amazing. have not been soo clearheaded in a long time. Please see the video (grizzly) and than go to mastercare.nl and get you colon cleanse
Detox Body Toxins
Length: 183
Rating: 3.90 (38 ratings)
Tags: mucous.colon cleanse Stomach Mucous colon parasite raw food health sex sexy fun medicine physic
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Four Horsemen: Plaque
http://www.rondo.blog.pl
http://www.ilustracje.info
full view: http://www.deviantart.com/deviation/61228316/
opencanvas/photoshop/volito2
music1: hitman2 maintitle by Jesper Kyd
music2: Larsen "Rebirth"
music3: from hitman2 by Jesper Kyd
music4: Raiders by Mark Morgan from fallout soundtrack
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Length: 570
Rating: 4.80 (26 ratings)
Tags: plaque apocalypse horsemen horse dead digital painting CG digart deviant photoshop opencanvas
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Easy Clay Project, Relief Plaque Pt1
Clay demo using a cut slab design. Decorative elements are added in the form of shapes, letters, symbols to express something personal about you. Tools include canvas, fettling knife, and clay stamps that were made earlier. see video on decorator stamps.
Additional art projects for home and school can be found at this web site. www.friendlyarts.net/Asdo/
Length: 600
Rating: 5.00 (1 ratings)
Tags: clay tools hand built design pattern stamps applicque' symbol
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